Optimum Ph For Alkaline Phosphatase Activity Soil' title='Optimum Ph For Alkaline Phosphatase Activity Soil' />Extracellular alkaline phosphatase from the filamentous fungus.Aspergillus caespitosus Purification and biochemical characterization.Among 3. 0 species of filamentous fungi isolated from Brazilian soil,Aspergillus caespitosus produced and secreted the highest levels of alkaline phosphatase in culture medium supplemented with xylan.The extracellular alkaline phosphatase was purified by DEAE cellulose and concanavalin A sepharose chromatography.The enzyme was a glycoprotein containing up to 5.Da, according to gel filtration in Sepharose CL 6.EXTRACELLULAR ALKALINE PHOSPHATASE FROM A.The enzyme was purified to homogeneity by a twostep chromatographic procedure data not.Effects of temperature, pH, and UV radiation on alkaline phosphatase activity in the terrestrial cyanobacterium Nostoc flagelliforme.B, and 5. 7 k. Da according to SDS PAGE.Non denaturing electrophoresis 6 PAGE of the purified enzyme produced a single band, suggesting that the native enzyme was a homodimer.Optima of temperature and p.H were 7. 5 C and 8.The enzyme was stable at 5.C and its activity was enhanced by 9.Mg. 2 1 mmolL. Nitrophenyl phosphate was the preferentially hydrolyzed substrate with.Km andvlim values of 7.L and 2. 85 mols, in the absence, and 9.L and 4. 18 mols, in the presence of Mg.The enzyme also hydrolyzed other phosphorylated amino acids O phosphothreonine,O phosphotyrosine,O phosphoserine.JulySeptember 2017 Volume 13 Issue 51 Page Nos.Online since Wednesday, July 19, 2017 Accessed 19,094 times.PDF access policy Full text access is free in.Professor Ravi Naidu Staff Profile The University of Newcastle, Australia.Professor Ravi Naidu is a global leader in contamination studies, studying agricultural and industrial impacts on the environment.His research has led to the implementation of policy directives for governments and new technology to manage and remediate polluted groundwater and soil, both in Australia and abroad.Ravi is also the Managing Director of the Cooperative Research Centre for Contamination, Assessment and Remediation of the Environment CRC CARE, an independent organisation that performs research, develops technologies and provides policy guidance for assessing, cleaning up and preventing contamination of soil, water and air.At the University of Newcastle, Ravi leads the Global Centre for Environmental Remediation GCER, where he continues to implement his vision to safeguard the environment for future generations.Shaping the world.As science speeds forward, Ravi acknowledges that public policy doesnt always keep up.With more than two decades working in the field of contaminants, including an esteemed chief investigator role with Australias Commonwealth Scientific and Industrial Research Organisation CSIRO, Ravi has made extensive contributions to national and global paradigms of risk assessment and remediation of contaminated sites.I have spent a lot of time developing a consensus amongst industry, regulators, Environmental Protection Authorities and researchers relating to contaminants, Ravi points out.As well as to remediate sites, a lot of work is required on the policy front.When I began my career, there was no remediation industry my work has led to an exponential increase in the number of people working in remediation, Ravi states humbly.This industry is now worth 3 billion dollars annually and employs thousands of people.A systems based approach to risk.Focusing on two broad categories, Ravis meticulous analyses investigate contaminants from agriculture and industry and their relationships with receptors.The receptor could be the environment, or human, Ravi says.My investigations look at the presence of contaminants in the environment, how they interact in the environment, and the route or path they take to receptors.Initially the focus of our work was on agriculture, Ravi observes.But many of the industrial activities over the past 5.Now the focus of our investigations to a very large extent is industrial contaminants and this is largely driven by the industries themselves.Ravis approach to this work has been to adopt whole of system inquiries to ensure the risks of contaminants are fully assessed and managed, whether they are in soil, groundwater or the urban environment.When assessing a contaminated site you assess the receptors, what the pathways to those receptors are, and what needs to be done to minimise exposure, Ravi confirms.Ravi notes the importance of this, given the severe health effects of some contaminants such as asbestos, arsenic, cadmium and mercury.The Professors approach is seeing GCER develop a new model in conjunction with CRC CARE to quantify risk posed by on site contaminants.Until recently, the predictive tool that has been used to assess risk and which is still being used is a database based on a lot of assumptions.Whenever you use assumptions, the output from the predictive tool can be quite conservative, and conservatism means you might end up remediating sites which do not require it.This new model will minimise uncertainties and save a lot of money for industries.CRC CARE and GCER caring and sharing knowledge.CRC CARE began in 1.Ravi initiated dialogue with industries and government departments dealing with contaminants.It took four years for industries to appreciate there was a need for a national Centre of Excellence to become a one stop shop for contaminants.The Centre, which brings together major companies, government departments and Environmental Protection Authorities, was established in 2.Commonwealth and is on track to become a Centre of Excellence beyond the year 2.In 2. 01. 5, the University of Newcastle invited Ravi to be Global Innovation Chair and Director of GCER.GCER reaches organisations in Australia and beyond from a capacity building perspective, Ravi notes.This entails training students as well as developing solutions for complex contamination problems.CRC CARE and GCER are safeguarding a clean environment for future generations, Ravi attests. 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Satisfaction is knowing you have helped train people who are able to clean up the mess we have created and prevent future degradation of our environment.Technology targeting toxins.Ravi has patented seven technologies to safeguard the environment and human health, including a product to remediate the toxic chemicals left behind by firefighting foams.No technology was available to help clean up wastewater and impacted soil, generated by firefighters when they train, Ravi declares.CRC CARE developed a new patented technology to fix this, utilising a naturally occurring material modified with a nontoxic chemical.This product can irreversibly and selectively capture toxic substances present in wastewater.Thus, the water that comes out is clean because of our filtration processes.The technology is being scaled up for field applications with plants operating at a number of remediation sites.Proactive Global Research.Working globally, CRC CAREs centre in China is enabling Ravi to collaborate with a number of scientists and engineers, to train locals to develop solutions for environmental contamination problems.We have developed a technology, named poo.CARE, which helps convert piggery waste into biogas, Ravi laughs.We have a very strong network in the region and our vision is to extend our Centres to other countries.The only way to move forward is to build the capacity in these countries, particularly developing nations, to deal with contamination issues.
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